FIDELIO GERARDO DANIEL
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Congresos y reuniones científicas
Congresos y reuniones científicas
Título:
A lectin and phospholipases A2 from Bothrops diporus venom: identification and sequencing by mass spectrometry
Autor/es:
YUNES QUARTINO PJ.,; PORTELA M.,; LIMA A., ; DURAN R.,; FIDELIO G.D.
Lugar:
VILLA CARLOS PAZ, CORDOBA
Reunión:
Congreso; XLII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE BIOFISICA; 2013
Institución organizadora:
SOC ARG DE BIOFISICA
Resumen:
We report protein sequences from lipolitic fractions of venom of Bothrops diporus, a viper from Argentina known as Yarará Chica. We have previously cloned two phospholipases and produce them via heterologous expression and subsequent renaturation (1). By similarity and identity to public database sequences, we classified these as type A2 (PLA2). In the present work we used conventional chromatographic techniques coupled to MALDI-TOF-TOF mass spectrometry analysis to prove the existence at the protein level of the cloned sequences and portions of new PLA2 isospecies. Along this work we also found a lectin dimer for which it is proposed the disulfide intermolecular connectivity
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