Juvenile hormone mediates lipid storage in the oocytes of Dipetalogaster maxima

RAMOS, FABIAN O.; LEYRIA, JIMENA; NOUZOVA, MARCELA; FRUTTERO, LEONARDO L.; NORIEGA, FERNANDO G.; CANAVOSO, LILIAN E.

INSECT BIOCHEM. MOLEC. BIOL.

ELSEVIER

Año: 2020 vol. 133

0965-1748

riatomines are vectors of Chagas disease and important model organisms in insect physiology. Kissing bugs are obligatory hematophagous insects. A blood meal is required to successfully complete oogenesis, a process primarily controlled by juvenile hormone (JH). We used Dipetalogaster maxima as an experimental model to further understand the roles of JH in the regulation of vitellogenesis and oogenesis. A particular focus was set onthe role of JH controlling lipid and protein recruitment by the oocytes. The hemolymph titer of JH III skipped bisepoxide increased after a blood meal. Following a blood meal there were increased levels of mRNAs in the fat body for the yolk protein precursors, vitellogenin (Vg) and lipophorin (Lp), as well as of their protein products in the hemolymph; mRNAs of the Vg and Lp receptors (VgR and LpR) were concomitantly up-regulated in theovaries. Topical administration of JH induced the expression of Lp/LpR and Vg/VgR genes, and prompted the uptake