Role of lipid transfer particle in delivery of diacylglycerol from midgut to lipophorin in larval Manduca sexta.

CANAVOSO, LE; WELLS, MA

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2001 vol. 31 p. 783 - 783

font size="2" face="Times-Roman"> The present work analyzed the function of lipid transfer particle (LTP) in the process of exporting diacylglycerol from larval Manduca sexta midgut cells to lipophorin. When midgut sacs, which had been prelabeled in vivo with [3H]oleic acid, were incubated in vitro with a lipophorin-containing medium, a significant amount of radiolabeled diacylglycerol was transferred to lipophorin. Negligible amounts of diacylglycerol were released into lipophorin-free medium. In contrast, lipid-labeled lipophorin did not transfer diacylglycerol to the midgut sacs. The transfer of diacylglycerol from