CANAVOSO LILIAN ETELVINA
Congresos y reuniones científicas
Título:
BIOCHEMICAL AND MOLECULAR ASPECTS OF VITELLOGENIN AND ITS RECEPTOR DURING THE REPRODUCTIVE CYCLE OF Dipetalogaster maxima
Autor/es:
LEYRIA, J; AGUIRRE, SA; NAZAR, M; CANAVOSO LE
Lugar:
La Falda, Cordoba
Reunión:
Jornada; XIX Reunion Anual de la Sociedad de Biología de Córdoba; 2013
Institución organizadora:
Sociedad de Biología de Córdoba
Resumen:
In insect females, vitellogenesis is a central event of reproduction
that elicits a massive synthesis of yolk protein precursors in the fat
body, mainly vitellogenin (Vg).Vg is taken up by developing oocytes
by receptor mediated endocytosis and stored as vitellin (Vt) in
yolk bodies. Unfavorable nutritional conditions may impair Vg
uptake by oocytes to favor follicle degeneration to an atretic stage.
The aim of this work was to better understand the roles of Vg and
its receptor, RVg, through the reproductive cycle of Dipetalogaster
maxima, a vector of Chagas? disease. The experimental approaches
included western blot, ELISA, RT-qPCR and in vivo Vg-FITC
uptake assays. The expression of Vg in the fat body and the stores
of Vt in the ovaries increased during vitellogenesis, in correlation
with an active process of Vg uptake by developing oocytes. By
contrast, Vg synthesis and uptake diminished at early follicular
atresia until it was undetectable in late atretic stages. It was shown
that the levels of RVg transcript were significantly high during
vitellogenesis, although RVg was also expressed during follicular
atresia. At present, our results strongly suggested that RVg may
play an important regulatory role in the initiation and progression
of follicular atresia.
that elicits a massive synthesis of yolk protein precursors in the fat
body, mainly vitellogenin (Vg).Vg is taken up by developing oocytes
by receptor mediated endocytosis and stored as vitellin (Vt) in
yolk bodies. Unfavorable nutritional conditions may impair Vg
uptake by oocytes to favor follicle degeneration to an atretic stage.
The aim of this work was to better understand the roles of Vg and
its receptor, RVg, through the reproductive cycle of Dipetalogaster
maxima, a vector of Chagas? disease. The experimental approaches
included western blot, ELISA, RT-qPCR and in vivo Vg-FITC
uptake assays. The expression of Vg in the fat body and the stores
of Vt in the ovaries increased during vitellogenesis, in correlation
with an active process of Vg uptake by developing oocytes. By
contrast, Vg synthesis and uptake diminished at early follicular
atresia until it was undetectable in late atretic stages. It was shown
that the levels of RVg transcript were significantly high during
vitellogenesis, although RVg was also expressed during follicular
atresia. At present, our results strongly suggested that RVg may
play an important regulatory role in the initiation and progression
of follicular atresia.
