Inhibition of iodide uptake and thyroid specific genes mRNA expresión by nitric oxide/guanylate cyclase pathway in FRTL-5 cells

BAZZARA LG; COSTAMAGNA ME; CABANILLAS AM; VÉLEZ ML; FOZZATTI L; PELLIZAS CG; MASINI-REPISO AM

mary Ann Liebert

Lugar: New York; Año: 2007 vol. 17 p. 117 - 117

bjective: Nitric oxide (NO) induces morphological and functional alterations in primary cultured thyroid cells. The aim of this paper was to analyze the direct influence of a long-term exposition to NO on parameters of thyroid hormone biosynthesis in FRTL-5 cells. Design: Cells were treated with the NO donor sodium nitroprusside (SNP) for 24–72 h. Main Outcome: SNP (50–500 mmol=L) reduced iodide uptake in a concentration-dependent manner. The inhibition of iodide uptake increased progressively with time and matched nitrite accumulation. SNP inhibited thyroperoxidase (TPO) and thyroglobulin (TG) mRNA expression in a concentration-dependent manner. SNP enhanced 3 0 ,5 0 -cyclic guanosine monophosphate (cGMP) production. 3 0 ,5 0 -cyclic adenosine phosphate (cAMP) generation was reduced by a high SNP concentration after 48 h. 8-Bromoguanosine 3 0 ,5 0 -cyclic monophosphate (8-Br-cGMP), a cGMP analog, inhibited iodide uptake as well as TPO and TG mRNA expression.