Thyroid hormone receptor alpha 1-beta 1 expression in epididymal epithelium from euthyroid and hypothyroid rats.

DE PAUL AL; MUKDSI JH; PELLIZAS CG; MONTESINOS MM; GUTIERREZ S; SUSPERREGUY S; DEL RÌO A; MALDONADO CA; TORRES AI

Springer

Año: 2008 vol. 129 p. 631 - 631

he objectives of the present work were to assess whether epithelial cells from the diVerent segments of epididymis express TR alpha1-beta1 isoforms, to depict its subcellular immunolocalization and to evaluate changes in their expression in rats experimentally submitted to a hypothyroid state by injection of 131I. In euthyroid and hypothyroid groups, TR protein was expressed in epididymal epithelial cells, mainly in the cytoplasmic compartment while only a few one showed a staining in the nucleus as well. A similar TR immunostaining pattern was detected in the diVerent segments of the epididymis. In hypothyroid rats, the number of TR-immunoreactive epithelial cells as well as the intensity of the cytoplasmic staining signiWcantly increased in all sections analyzed. In consonance to the immunocytochemical analysis, the expression of TR alpha1-beta1 isoforms, assessed by Western blot revealed signiWcantly higher levels of TR in cytosol compared to the nuclear fractions. F