Bacterial lipopolysaccharide increases thyroglobulin gene expression involving TTF-1 and Pax8 transcription factors

VELEZ ML; COSTAMAGNA E; KIMURA E; FOZZATTI L; PELLIZAS CG; MONTESINOS MM; LUCERO AM; COLEONI AH; SANTISTEBAN P; MASINI-REPISO AM

ENDOCRINOLOGY

HighWire Press

Año: 2006 vol. 147 p. 3270 - 3270

0013-7227

b> The bacterial lipopolysaccharide (LPS) is a biological activator that induces expression of multiple genes in several cell types. LPS has been proposed as an etiopathogenic agent in autoimmune diseases. However, whether LPS affects the expression of autoantigens has not been explored. Thyroglobulin (TG) is a key protein in thyroid hormonogenesis and one of the major thyroid autoantigens. This study aimed to analyze the action of LPS on TG gene expression in Fisher rat thyroid cell line FRTL-5 thyroid cells. We demonstrate that LPS increases the TSH-induced TG protein and mRNA level. Evidence that the effect of LPS is exerted at the transcriptional level was obtained by transfecting the minimal T