Cooperative nongenomic and genomic actions on thyroid hormone mediated-modulation of t cell proliferation involve up-regulation of thyroid hormone receptor and inducible nitric oxide synthase expression

BARREIRO-ARCOS, M; STERLE, H; PAULAZO, M; VALLI, E; KLECHA, A; ISSE, B; PELLIZAS, C; FARÍAS, R; CREMASCHI, G

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2011 vol. 226 p. 3208 - 3208

he surface balance technique was employed to study the interactions of 3,5,3´,5´ tetraiodo L-thyronine, 3,5,3´ triiodo L-thyronine, and 3,5-diiodothyronine with monomolecular phospholipid monolayers spread at the air-water interface. With this technique the insertion of thyroid hormones into egg yolk phosphatidylcholine was investigated. An increase of surface pressure and a substantial decrement in surface potential were observed after the injection of these hormones beneath a phospholipid monolayer. The negative dipole contribution upon hormone interaction opposes the well-known positive contribution of phospholipids. These effects correlated with iodo content of the thyroid molecule analogues 3,5,3´,5´ tetraiodo L-thyronine >3,5,3´ triiodo L-thyronine >3,5-diiodothyronine. To our knowledge, these observations suggest a new and surprising effect of thyroid hormones on the regulation of transmembrane dipolar organization