Thyroid tumor cell-fibroblast interaction produces extracellular vesicles that increase fibroblast proteolytic activity

BRAVO MIANA R; DELLA VEDOVA AB; DE PAUL AL; REMEDI MM; GUANTAY ML; PELLIZAS CG; DONADIO AC

Endocrine Connections

EC Bioscientifica

Lugar: berlin; Año: 2020 vol. 9 p. 506 - 506

2049-3614

xtracellular vesicles (EVs) are protagonists in cell-cell communication. Despite thyroidcancer being the most common endocrine malignancy, the contribution of the tumormicroenvironment to thyroid cancer progression is still largely underexplored. We focusedon the role of thyroid tumor cell-fibroblast interaction and EVs as mediators of tumorstromainterplay, in the promotion of thyroid tumor aggressiveness. Thyroid tumor (TPC-1,8505c) or non-tumor thyroid cells (NThyOri) were co-cultured wi th human fibroblasts (Fb).Thyroid cell migration was investigated by the wound-healing assay and actin-networkstaining. Cell-CD147 expression was characterized by flow cytome try. EVs, obtained byultracentrifugation of conditioned media (CMs), were characterized by transmissionelectron-microscopy and CD81 and CD147 expression. Metalloproteinases (MMPs) wereevaluated by zymography in CMs. A migratory phenotype was triggered in thyroid tumorcells treated with CMs from Fb or from Fb-thyroid tumor cell