Polysaccharide extracts from mycelia of Ganoderma australe: effect on dendritic cell immunomodulation and antioxidant activity

GALLO, A.L.; SOLER, F.; PELLIZAS, C.; VÉLEZ, M.L.

Journal of Food Measurement and Characterization

Springer

Lugar: Berna; Año: 2022 vol. 16 p. 3251 - 3251

2193-4126

any fungi have inner-polysaccharides (IPS) that can modulate the immune response and/or have antioxidant activity. Ganoderma australe, a species from Patagonia, is a likely candidate to produce bioactive IPS. Our aims were to assess the ability of its IPS to induce maturation and activation of dendritic cells (DCs), to determine their antioxidant activity, and to evaluate the influence of the chemical composition on the bioactivity of the IPS. Mycelia were cultivated in different liquid culture media (M1, M2, and AG), and polysaccharides were extracted with hot water. Mice bone marrow-derived DCs were exposed to the different IPS, and the percentage of cells expressing CD86, MHC II, and IL-12 was determined. The antioxidant activity was evaluated through four assays. Results showed that the chemical composition of the three extracts was different. Glucose, mannose, and galactose prevail in all the extracts, although in different proportions. IPS from mycelia growing in M1 (IPS_M1) sig