Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure

MARTÍNEZ, SOL ROMINA; AIASSA, VIRGINIA; SOLA, CLAUDIA; BECERRA, MARÍA CECILIA

Journal of Global Antimicrobial Resistance

Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer

Lugar: Amsterdam; Año: 2020

2213-7165

ACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens multi-resistant to antimicrobial agents (AA). The AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported.OBJECTIVE:The role of the oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of Linezolid (LZD) on S. aureus strains.METHODS:Oxidative stress markers, such as superoxide dismutase enzyme (SOD) activity, the global antioxidant response, the advanced oxidation protein products (AOPP) and the basal levels of glutathione in 28 clinical and 2 reference strains were performed.RESULTS AND CONCLUSIONS:We identified 10 of 30 strains showing a slightly increase in reactive species under LZD treatment with respect to the untreated control (between 22 to 56%). The higher generation was detected in the clinical strains compared to