SOLA CLAUDIA DEL VALLE
Congresos y reuniones científicas
Título:
METHICILLIN-RESISTANT Staphylococcus aureus BELONGING TO CC121 RESPONSIBLE FOR INVASIVE INFECTIONS
Autor/es:
EGEA ANA LIA; GONZALEZ L; MOYANO A.J; BOCCO JL; SOLA C
Lugar:
Potrero de los Funes, San Luis
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y. Biología Molecular (SAIB-2011).; 2011
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y. Biología Molecular
Resumen:
Since 1990s methicillin resistant Staphylococcus aureus
(MRSA)emerged as a community-associated pathogen
(CAMRSA) worldwide. Most CAMRSA carry the staphylococcal
cassette chromosome (SCCmec) type IV and the Panton-Valentine
leukocidin (PVL) gene. Successful lineages of MSSA PVL+ would
behave as a reservoir for emergence of CAMRSA PVL+ clones.
Although the CC121 is one of most dominant MSSA lineages, its
genetic background seems to be incompatible for stable integration
of SCCmec. The aim of this work was the molecular and clinical
analysis of two cases of CAMRSA infections caused by strains
belonging to the lineage CC121 by molecular typing methods.
These strains were isolated from children (11 and 14 years old)
hospitalized in a tertiary care public hospital in Córdoba. They
were affected by osteomielitis with torpid evolution; one of them
was complicated with sepsis. Molecular analysis of the strains
showed that both belong to CC121 characterized by: Pulsotype U-
ST1210 (CC121), agr 4, SCCmecIVa-spat645. They harbor the
following genes pvl, Enterotoxin B, cluster egc, adhesins for bone
sialoprotein (bpp) and for collagen (cna). We demonstrated the
emergence of two MRSA isolates PVL+ belonging to CC121,
associated to SCCmecIVa. As far as we know, this genetic
background has never been reported in MRSA, moreover this clone
have all the genetic potential to becoming an epidemic CAMRSA.
(MRSA)emerged as a community-associated pathogen
(CAMRSA) worldwide. Most CAMRSA carry the staphylococcal
cassette chromosome (SCCmec) type IV and the Panton-Valentine
leukocidin (PVL) gene. Successful lineages of MSSA PVL+ would
behave as a reservoir for emergence of CAMRSA PVL+ clones.
Although the CC121 is one of most dominant MSSA lineages, its
genetic background seems to be incompatible for stable integration
of SCCmec. The aim of this work was the molecular and clinical
analysis of two cases of CAMRSA infections caused by strains
belonging to the lineage CC121 by molecular typing methods.
These strains were isolated from children (11 and 14 years old)
hospitalized in a tertiary care public hospital in Córdoba. They
were affected by osteomielitis with torpid evolution; one of them
was complicated with sepsis. Molecular analysis of the strains
showed that both belong to CC121 characterized by: Pulsotype U-
ST1210 (CC121), agr 4, SCCmecIVa-spat645. They harbor the
following genes pvl, Enterotoxin B, cluster egc, adhesins for bone
sialoprotein (bpp) and for collagen (cna). We demonstrated the
emergence of two MRSA isolates PVL+ belonging to CC121,
associated to SCCmecIVa. As far as we know, this genetic
background has never been reported in MRSA, moreover this clone
have all the genetic potential to becoming an epidemic CAMRSA.
