Promising chitosan coated alginate-tween 80 nanoparticles as rifampicin co-administratd ascorbic acid delivery carrier against Mycobacterium tuberculosis

SCOLARI ROMINA; PÁEZ, PAULINA L.; MARIELA SÁNCHEZ BORZONE; GRANERO GLADYS

AAPS PHARMSCITECH

SPRINGER

Lugar: Berlin; Año: 2018

1530-9932

he aim of this study was to design a nanocarrier system for inhalationdelivery of rifampicin (RIF) in combination with ascorbic acid (ASC) namelyconstituted of sodium alginate coated with chitosan and tween 80 (RIF/ASC NPs)as a platform for the treatment of pulmonary tuberculosis infection. A Box Behnkenexperimental design and response surface methodology (RSM) were applied toelucidate and evaluate the effects of several factors on the nanoparticle´sproperties. On the other hand, it was found that RIF/ASC NPs were less cytotoxicthan the free RIF, showing a significant improved activity against 9 clinical strainsof Mycobacterium tuberculosis (M. tb) in comparison with the free drug. RIF/ASCNPs had an average particle size of 324.0 ± 40.7 nm, a polydispersity index of0.226 ± 0.030 and a zeta potential of -28.52 ± 0.47 mV and the surface washydrophilic. The addition of sucrose (1% w/v) to the nanosuspension resulted in theformation of a solid pellet easily redispersible after lyophilizat