Exploring the Toxicity, Lung Distribution, and Cellular Uptake of Rifampicin and Ascorbic Acid-Loaded Alginate Nanoparticles as Therapeutic Treatment of Lung Intracellular Infections

SCOLARI ROMINA; VOLPINI XIMENA; FANANI MARÍA LAURA; DE LA CRUZ-THEA BENJAMÍN; NATALI LAUTARO; MUSRI, M. M.; GRANERO GLADYS

MOLECULAR PHARMACEUTICS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2021

1543-8384

anotechnology is a very promising technological toolto combat health problems associated with the loss of effectiveness ofcurrently used antibiotics. Previously, we developed a formulationconsisting of a chitosan and tween 80-decorated alginate nanocarrierthat encapsulates rifampicin and the antioxidant ascorbic acid (RIF/ASC), intended for the treatment of respiratory intracellularinfections. Here, we investigated the effects of RIF/ASC-loadedNPs on the respiratory mucus and the pulmonary surfactant. Inaddition, we evaluated their cytotoxicity for lung cells in vitro, andtheir biodistribution on rat lungs in vivo after their intratrachealadministration. Findings herein demonstrated that RIF/ASC-loadedNPs display a favorable lung biocompatibility profile and a uniformdistribution throughout lung lobules. RIF/ASC-loaded NPs were mainly uptaken by lung macrophages, their primary target. Insummary, findings show that our novel designed RIF/ASC NPs could be a suitable system for antibioti