Incorporation of Carbamazepine, a non-ionic drug, in Layered Double Hydroxides

Congreso; Incorporation of carbamazepine, a non-ionic drug, in layered Double Hydroxides; 2019

Layered Double Hydroxides (LDHs) are inorganic solids composed of cationic layers and inter-lamellar spaces filled with negative ions. In the last years, LDHs have gain interest as carriers of anionic drugs, which replace the inter-layers ions. Most anti-tumor drugs are systemic and these produce many side effects. LDHs could protect them and avoid their activity until they arrive to the tumor; however, most anti-tumor drugs have no charge and then are not able to enter into the inter-lamellar space. The use of a surfactant was used in this work to overcome this problem. Carbamazepine (CBZ), an anticonvulsant with a formal charge of 0, have shown efficacy against some solid cancer cell lines; it also produces alterations in red blood cells. This drug was used as a reference to probe the system. CBZ was first incorporated in micelles of sodium cholate, a surfactant with negative charge. This complex was then incorporated in HDLs composed of Mg2-Al-NO3 by different methods: ionic exchange, co-precipitation and reconstruction. The methods were also tested with different amounts of surfactant, at a critical micelle concentration. X ray powder diffractograms showed that the drug incorporated in the system when synthetized by ionic exchange and reconstruction, but not by co-precipitation. The dimension of the HDLs with the incorporation of the drug in the micelles was measured by Dynamic Light Scattering. The amount of CBZ loaded in the system was determined by UV and found to be ~2 %, which represents almost the 100 % of the original amount of drug. Assays of drug released were done in Franz Cells with a Simulated Body Fluid (pH 7.4) and an Acetate buffer (pH 4.8) in order to analyze the protector roll of the system in the blood and the drug release inside the tumor cells respectively.The system analyzed in this work increase the spectrum of use of LDHs as drug delivery carriers by allowing the incorporation of all kind of drugs and not only of that with anionic charge.