Effects of thymus supernatants on the phenotype of different subsets of

MACCIONI M; ROMERO-PIFFIGUER M; RIVERO VE; RIERA CM; FERRO ME

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1994 vol. 41 p. 1 - 1

eritoneal cells (PC) obtained 2 h subsequent to intraperitoneal (i.p.) injectionof low doses (200 micrograms) of a purified fraction of rat male accessory glands(FI-RAG) are phenotypically and functionally different from those obtained 24 hafter i.p. injection. In fast, PC obtained 2 h after FI-RAG injection are mainly IE+ and are involved in inducing specific suppression to RAG. In contrast, PCobtained 24 h after FI-RAG injection are mainly IA+ and capable of inducingspecific response to RAG. For their induction, IA+ PC require cells within orrecently derived from bone marrow. In order to analyze the mechanisms involved inIA+ bone marrow-dependent cell generation in the peritoneum, we studied thedistribution of FI-RAG following i.p. injection. It was established that FI-RAGis found mainly in the thymus 2 h after injection and remains there for at least 24 h. Subsequently, we analyzed, in 4 groups of rats, the influence