Autoantibodies against rat prostate antigens. Association of specific IGG2b and IGG2c with the DTH response

MACCIONI M; RIVERO VE; RIERA CM

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 1996 vol. 9 p. 485 - 485

div> Although autoimmune response against most tissues in the body has been extensively described, very little is known about autoimmune response against prostate antigens either in humans or animals. In this work we studied the autoimmune response elicited against rat prostate (RP) in Wistar rats immunized with a chemically modified extract of syngeneic male sex accessory glands (MRAG). We observed that one immunization was enough for the induction of positive delayed-type hypersensitivity test (DTH), which was higher on day 15 than on day 30. It was also enough to induce IgG autoantibodies to RP although a second injection was necessary to obtain a more frequent occurrence and a greater reactivity. The autoantibodies against RP were directed mainly to the cytosolic fraction and reacted at least with two molecules of 43 and 20 KD. Sera obtained on days 30 and 45 showed presence of specific IgG, IgA and IgM. Specific IgG2b and IgG2c were found on both days. On day 30 none of the s