RIVERO VIRGINIA
Congresos y reuniones científicas
Título:
IL10 PRODUCING B LYMPHOCYTES IN AN EXPERIMENTAL MODEL OF CHLAMYDIA MURIDARUM INFECTION
Autor/es:
SANCHEZ LEONARDO; GODOY GJ,; GOROSITO M, ; GRUPPI A, ; MOTRICH RD; RIVERO VE
Lugar:
Mar del Plata
Reunión:
Congreso; LIX REUNIÓN CIENTÍFICA ANUAL de la SAIC- LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2014
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
Chlamydia trachomatis (CT) causes an important number of genital tract infections worldwide. Although after the infection a specific immune response is induced, some data suggest that its quality and/or intensity would not be adequate since disease is often persistent and recurrent. It has been postulated that Chlamydia would evade host defenses through different strategies, such as the induction of anti-inflammatory cytokines like IL10 and TGFß. Herein, we aimed to evaluate the role of IL10 after male genital tract infection with Chlamydia muridarum (Cm), a serovar that infects mice and produces a similar disease to that observed in humans. Cytokine production by splenocytes (MNC) from non-infected mice incubated with Cm was analyzed. MNC in vitro stimulated with heat inactivated Cm mostly secrete IL10 (IL10: 920±80pg/ml; IL6: 245±2 pg/ml; TNF: 130±20 pg/ml; IFN: 245 ± 2 pg/ml). FACS analyses revealed that the main producers of IL10 are CD45+CD19+ cells. Accordingly, magnetic beads purified B lymphocytes produced high levels of IL10 after in vitro stimulation with Cm. Experiments in which NOD mice were inoculated with Cm in the meatus urethra and then euthanized at different times post infection (pi) showed that MNC from infected mice produce the highest levels of IL10 at early times pi. Indeed, higher levels of IL10+CD45+CD19+ cells were found at 4 day pi, accompanied with low levels of IL10+CD45+CD3+ cells. Experiments using NOD and NOD-SCID mice showed a significant reduction in the IL10 amounts produced by MNC from infected NOD-SCID when compared to those from infected NOD mice (p<0.05), supporting the idea that adaptive immune cells are the main producers of IL10. These results suggest that Cm has the ability to induce IL10 production by B lymphocytes at early times after infecting the host. This IL10 production could be related to the poorly protective immune responses observed in infected subjects and with the chronic nature of CT genital tract infection.