Prevalence of Human Papilloma Virus in male partners of infertile couples. Semen quality and coinfection analysis

OLIVERA C; CUFFINI C; TISSERA A; MOLINA R; RIVERO VE

Barcelona

Congreso; The 33th International Papillomavirus Conference & Basic Science, Clinical and Public Health Workshops; 2020

INTERNATIONAL PAPILLOMAVIRUS SOCIETY (IPVC).

INTRODUCTION: Previous studies revealed that HPV was more prevalent in infertile subjects compared to fertile ones. Some studies associate HPV infection with reduced mobility and sperm viability, while others do not report these alterations. Most of these studies do not analyze the concomitant presence of other STI associated with infertility. METHODS: 143 male partners of infertile couples were analyze to evaluate genital tract HPV infection prevalence and its correlation with sperm parameters. Samples consisted of a mixture of semen and urethral swab. HPV was analyzed by PCR method and genotyped by RFLP analysis. The presence of Chlamydia trachomatis, Ureaplasma urealiticum, Mycoplasma hominis, HSV1 and HSV2 was also analyzed. Seminal quality was evaluated according to WHO 2010 standard.3) RESULTS: We found that 26,3 % of the samples were positive for HPV. The most prevalent genotype found was genotype 6. Others low risk genotypes found were 11 and 72. Oncogenic and possibly oncogenic HPV (31, 16, 18, 82 and 53) were detected in 31.25% of positive samples. 62.5% of the patients HPV positive had co-infection with at least one of the other pathogens analyzed. The HPV+ patients did not show specific alterations in the seminal parameters with respect to the HPV- group. We do not found presence of leukocyspermia associated with HPV infection4) CONCLUSIONS This study provide the first Argentinean estimate of genital HPV prevalence in male population of infertile individuals. The distribution of genotypes found was different from that of other geographical areas, highlighting the importance of local studies of HPV infection. The absence of leukocytospermia in HPV positive patients would indicate that the virus promotes a local anti-inflammatory environment. Evaluate other STI associated with infertility such as those studied in the present work could contribute to understand the real effect of HPV in seminal quality.