Regulation of membrane expansion at the nerve growth cone

K. H. PFENNINGER; L. LAURINO; D. PERETTI; L. LAURINO,; S. ROSSO; G. MORFINI; A. CÁCERES; S. QUIROGA

JOURNAL OF CELL SCIENCE

Año: 2003 vol. 116 p. 1209 - 1209

0021-9533

b> Exocytotic incorporation of plasmalemmal precursor vesicles (PPVs) into the cell surface is necessary for neurite extension and is known to occur mainly at the growth cone. This report examines whether this is a regulated event controlled by growth factors. The Golgi complex and nascent PPVs of hippocampal neurons in culture were pulse-labeled with fluorescent ceramide. We studied the dynamics of labeled PPVs upon arrival at the axonal growth cone. In controls and cultures stimulated with brain-derived neurotrophic factor (BDNF), PPV clusters persisted in growth cones with a half-life (t1/2