The Insulin-Like Growth Factor 1 Receptor Is Essential for Axonal Regeneration in Adult Central Nervous System Neurons

DUPRAZ, S.; DIEGO GRASSI; DIANA KARNAS; ALVARO NIETO GUIL; DAVID HICKS; SANTIAGO QUIROGA

PUBLIC LIBRARY SCIENCE

Año: 2013 vol. 8 p. 1 - 1

xonal regeneration is an essential condition to re-establish functional neuronal connections in the injured adult centralnervous system (CNS), but efficient regrowth of severed axons has proven to be very difficult to achieve. Althoughsignificant progress has been made in identifying the intrinsic and extrinsic mechanisms involved, many aspects remainunresolved. Axonal development in embryonic CNS (hippocampus) requires the obligate activation of the insulin-likegrowth factor 1 receptor (IGF-1R). Based on known similarities between axonal growth in fetal compared to mature CNS, wedecided to examine the expression of the IGF-1R, using an antibody to the bgc subunit or a polyclonal anti-peptideantibody directed to the IGF-R (C20), in an in vitro model of adult CNS axonal regeneration, namely retinal ganglion cells(RGC) derived from adult rat retinas. Expression of both bgc and the b subunit recognized by C20 antibody were low infreshly iso