Controlled lateral packing of insulin monolayers influences neuron polarization in solid-supported cultures

ERNESTO GRASSO; RAFAEL OLIVEIRA; MARIANA OKSDATH; SANTIAGO QUIROGA; BRUNO MAGGIO

ColloidSurfaceB

Elsevier

Lugar: Philadelphia; Año: 2013 p. 59 - 59

0927-7765

eurons are highly polarized cells, composed of one axon and several branching dendrites. One importantissue in neurobiology is to understand the molecular factors that determine the neuron to developpolarized structures. A particularly early event, in neurons still lacking a discernible axon, is the segregationof IGF-1 (Insulin like Growth Factor-1) receptors in one neurite. This receptor can be activatedby insulin in bulk, but, it is not known if changes of insulin organization as a monomolecular film mayaffect neuron polarization. To this end, in this work we developed solid-supported Langmuir–Blodgettfilms of insulin with different surface packing density. Hyppocampal pyramidal neurons, in early stage ofdifferentiation, were cultured onto those substrates and polarization was studied after 24 h by confocalmicroscopy. Also we used surface reflection interference contrast microscopy and confocal microscopyto study attachment patte