Eudragit E100(®) potentiates the bactericidal action of ofloxacin against fluoroquinolone-resistant Pseudomonas aeruginosa.

ROMERO V-; PONS P.; BOCCO J.L.; MANZO R.; ALOVERO F.

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 334 p. 102 - 102

e report the enhanced bactericidal activity of ofloxacin in drug-containingEudragit E100® dispersions (EuCl-OFX) against Pseudomonas aeruginosa andthe effect of the cationic polymer on bacterial membrane. Organisms treatedwith EuCl-OFX showed changes in cell morphology, altered outer membrane(OM) and cytoplasm with low electrodensity areas. Zeta potential of bacterialsurface was shifted to positive. Sensitization to lytic agents was also observed.A profound effect on bacterial size, granularity and membrane depolarizationwas found by flow cytometry. Cultures exposed to drug-free polymer alsoshowed some damaged bacterial membranes, but there was no significant celldeath. Inhibition of P. aeruginosa by EuCl-OFX may involve surface effect and,to some extent, permeation effect. The cationic polymer act to mitigate theelectronegativity of cell surface in the process of disorganizing the OM, render-ing it more permeable