RXR-alpha regulates the Pregnancy Specific beta-1-Glycoprotein 5 gene transcription through a functional Retinoic Acid Responsive Element.

LÓPEZ-DÍAZ FERNANDO; NORES, RODRIGO; PANZETTA-DUTARI, GRACIELA; SLAVIN, DANIELA; PRIETO, CLAUDIO; KORISTCHONER, NICOLAS; BOCCO JOSE LUIS

PLACENTA

Elsevier

Lugar: Orlando - USA; Año: 2007 vol. 28 p. 898 - 898

0143-4004

uman pregnancy-specific glycoproteins (PSG) are major placental polypeptides encoded by eleven highly conserved genes expressed by the syncytiotrophoblast. The minimal promoter region of all PSG genes contains a putative Retinoic Acid Responsive Element (RARE) though the ability of retinoids to regulate PSG gene expression has not been established. Retinoid signaling pathway plays a key role for overall placenta biology and is essential for trophoblast differentiation. In this work, we investigated the participation of the RARE motif in the regulation of PSG5 gene transcription by retinoic acid and its receptors. The minimal promoter region of PSG5 gene was activated by RXRalpha but not by RARalpha, in a ligand-dependent manner. The RARE sequence of PSG5 gene promoter was recognized by endogenous RXRalpha present in placental nuclear extracts as well as by RXRalpha either over expressed in cultured non-placental cells or in vitro translated. Mutations at specific nucleotides within th