Antibacterial and Cytotoxic Activity of Compounds Isolated from Flourensia oolepis.

JORAY MB; TRUCCO LD; GONZÁLEZ ML; NAPAL GN; PALACIOS SM; BOCCO J.L.; CARPINELLA MC

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2016

1741-427X

he antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 μg/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1?5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6?9.9 μM) and a lower effect against CML cells (IC50 = 27.5?30.0 μM). Flow cytometry was used to anal