Human Pregnancy Specific Glycoprotein 1a (PSG1a) induces alternative activation in human and mouse monocytes and suppress the accessory cells-dependent T cell proliferation.

MOTRAN C.; LOPEZ-DIAZ F.; GRUPPI A.; SLAVIN D.; CHATTON B.; BOCCO JL* (CORRESPONDIG AUTHOR)

FEDERATION AMER SOC EXP BIOL

Lugar: Bethesda; Año: 2002 vol. 72 p. 512 - 512

t has been proposed that pregnancy-specific factors induce the suppression of a specific arm of the maternal response accompanied by activation of the nonspecific, innate immune system. The aim of this study was to determine whether pregnancy-specific glycoprotein 1a (PSG1a), the major variant of PSG polypeptides, is able to modulate the monocyte/macrophage (Mo) metabolism to regulate T cell activation and proliferation. Using the recombinant form of this glycoprotein (rec-PSG1a), expressed in mammalian cells with a vaccinia-based expression vector, we have demonstrated that human PSG1a induces arginase activity in peripheral blood human Mo and human and murine Mo cell lines. In addition, rec-PSG1a is able to induce alternative activation because it up-regulates the arginase activity and inhibits the nitric oxide production in Mo activated by lipopolysaccharides. We also observed that rec-PSG1a is an important accessory cells-dependent T cell suppressor factor that causes partial grow