Genomic organization and functional analysis of the gene encoding the Krüppel-like transcription factor KLF6.

GEHRAU, RICARDO; D´ASTOLFO, DIEGO; PRIETO, CLAUDIO; BOCCO, JOSÉ LUIS; KORITSCHONER, NICOLAS

BIOCHIMICA ET BIOPHYSICA ACTA, N. GENE STRUCTURE AND EXPRESSION

ELSEVIER

Lugar: Amsterdam; Año: 2005 vol. 1730 p. 137 - 137

0167-4781

he Krüppel-like transcription Factor 6 (KLF6) is regulated during cell proliferation and differentiation events like mammalian development and tissue regeneration, while its aberrant expression is associated with tumor formation. To investigate KLF6 transcriptional control, the genomic organization of human KLF6 together with its cis-regulatory region was analyzed. A high sequence homology of KLF6 regulatory regions was found in mammals, which in turn predicts a high degree of evolutionary conserved transcriptional mechanisms. A transcription start site was identified at the first nucleotide downstream of a potential initiator element. Also, the role of KLF6 regulatory regions was determined by transfection experiments. A minimal promoter region lacking a TATA-box yet containing an Initiator was identified and found to be active in all cells analyzed. In addition, two strong activating sequences were located between positions -407/-344 and -307/-207, where the latter contained Sp1 and