Identification and molecular weight of SP1 synthesized from mRNA of human placenta in a wheat germ cell-free system.

BOCCO J.L.; ACTIS L.A.; FLURY A.; PATRITO LC.

MOLECULAR BIOLOGY REPORTS

SPRINGER

Lugar: Berlin; Año: 1986 vol. 12 p. 55 - 55

0301-4851

oly (A+)-mRNA obtained from human term placenta using guanidine HCl and oligo (dT) cellulose chromatography was translated in a wheat germ cell-free system. SDS-polyacrylamide gel electrophoresis analysis of the translation products revealed the presence of several polypeptides with molecular weights ranging from 10 KD to 70 KD. A single protein band representing around 1% of the total radioactive proteins synthesized in the presence of 2.5 micrograms of mRNA was isolated by immunoprecipitation, using specific antiserum against either the native ´Pregnancy-specific beta 1-glycoprotein´ or a reduced and carboxymethylated derivative. The molecular weight of 31-2 KD of this translation product corresponding to the nonprocessed precursor could account for the 43 KD value assigned to the protein purified form human pregnant serum.