Thyroid tumor cells-fibroblasts crosstalk: role of extracellular vesicles

BRAVO-MIANA, ROCÍO DEL CARMEN; DELLA VEDOVA, ANA BELÉN; DE PAUL, ANA LUCÍA; REMEDI, MARÍA MÓNICA; GUANTAY, MARÍA LAURA; GILARDONI, MÓNICA BEATRIZ; PELLIZAS, CLAUDIA GABRIELA; DONADIO, ANA CAROLINA

Endocrine Connections

Biocientifica

Lugar: Bristol; Año: 2020 vol. 9 p. 506 - 506

umor-stroma crosstalk leads to a tumor-promoting microenvironment. In this milieu, extracellular vesicles (EVs) are protagonists in cell-cell communication. Despite thyroid cancer being the most common endocrine malignancy, the contribution of the tumor microenvironment to thyroid cancer progression is still largely underexplored. We focused on the role of thyroid tumor cell-fibroblast interaction and EVs as mediators of tumor-stroma interplay, in the promotion of thyroid tumor aggressiveness. Thyroid tumor (TPC-1, 8505c) or non-tumor thyroid cells (NThyOri) were co-cultured with human fibroblasts (Fb). Thyroid cell migration was investigated by the wound-healing assay and actin-network staining. Cell-CD147 expression was characterized by flow cytometry. EVs, obtained by ultracentrifugation of conditioned media (CMs), were characterized by transmission electron-microscopy and CD81 and CD147 expression. Metalloproteinases (MMPs) were evaluated by zymography in CMs. A migratory phenotyp