IGF-1 regulates the extracellular level of active MMP-2 and promotes 2 Müller Glial Cell motility

LORENC VE; JALDIN-FINCATI J; LUNA JD; CHIABRANDO GA; SANCHEZ MC

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

ASSOC RESEARCH VISION OPHTHALMOLOGY INC

Lugar: Rockville, MD 20852; Año: 2015 vol. 56 p. 6948 - 6948

0146-0404

urpose. In ischemic proliferative retinopathies, Müller glial cells (MGCs) acquire migratory abilities. However, the mechanisms that regulate this migration remain poorly understood. In addition, proliferative disorders associated with enhanced activities of matrix metalloproteinases (MMPs) also involve insulin-like growth factor 1 (IGF-1) participation. Therefore, the main interest of this work was to investigate the IGF-1 effecton the extracellular proteolytic activity in MGCs.Methods. Cell culture supernatants and cell lysates of the human MGC line MIO-M1stimulated with IGF-1, were analyzed for MMP-2 by zymographic and Western blotanalysis. The MGCs motility was evaluated by scratch wound assay. MMP-2, β1-integrin and focal adhesions were detected by confocal microscopy. The localization of active MMPs and actin cytoskeleton were evaluated by in situ zymography.Results. IGF-1 induced the activation of canonical signaling pathways, through the IGF-1R phosphorylation. Culture su