Alpha2-Macroglobulin induces glial fibrillary acidic protein mediated by LRP1 in retinal Muller cells.

BARCELONA P; ORTIZ S; CHIABRANDO G; SANCHEZ MC

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

ASSOC RESEARCH VISION OPHTHALMOLOGY INC

Año: 2011 vol. 52 p. 778 - 778

0146-0404

URPOSE. Although it is known that Mu¨ller cells express theglial fibrillary acidic protein (GFAP) in response to acute retinaldamage, the regulatory mechanism is not completely understood. 2-Macroglobulin (2M) and its receptor, low-densitylipoprotein receptor-related protein 1 (LRP1), have also beenfound in injured retinas. Herein, the authors examined theinvolvement of the 2M/LRP1 system in GFAP expression inMu¨ller cells using in vitro and in vivo experimental models.METHODS. Using Western blot analysis and immunocytochemistry, the authors evaluated the effect of 2M* on GFAP expression in the Mu¨ller cell line MIO-M1, which constitutively expresses LRP1. Intracellular signaling pathways activated by2M* were examined by Western blot analysis. The effect of2M* on GFAP expression in the mouse retina was examinedby intravitreal microinjection of 2M* in mouse eyes.RESULTS. These data demonstrate that 2M* induced GFAP expression in the MIO-M1 cell line, which was selectivelyblocked by RAP