Guidelines for the use and interpretationof assays for monitoring autophagy

KLIONSKY DANIEL; AMAL KAMAL ABDEL-AZIZ; ABDELLATIF M,; SARA ABDELFATAH; ASGHAR ABDOLI ; STEFFEN ABEL; MARIA CECILIA SANCHEZ

AUTOPHAGY

LANDES BIOSCIENCE

Año: 2021

1554-8627

n 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect fo