Differential effects of insulin on peripheral diabetes-related changes in mitochondrial bioenergetics: involvement of advanced glycosylated end products

REMOR A; DE MATOS J; GHISONI K; EIDT G; BÚRIGO M; FABRO DE BEM A; LOCK SILVEIRA PC; DE LEÓN A; SANCHEZ MC; SARAIVA-GONÇALVES CA; QUINCOZES-SANTOS A; BORBA ROSA R; LATINI A

BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR BASIS OF DISEASE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011

0925-4439

arge scale clinical trials have demonstrated that an intensive antihyperglycemic treatment in diabetesmellitus (DM) in individuals reduces the incidence of micro- and macrovascular complications, e.g.nephropathy, retinopathy, DM-accelerated atherosclerosis, myocardial infarction, or limb amputations.Here, we investigated the effect of short- and long-term insulin administration on mitochondrial function inperipheral tissues of streptozotocin (STZ)-induced hyperglycemic rats. In addition, the in vitro effect ofmethylglyoxal (MG), advanced glycation end products (AGEs) and human diabetic plasma on mitochondrialactivity was investigated in skeletal muscle and liver mitochondria and in rat skin primary fibroblasts.Hyperglycemic STZ rats showed tissue-specific patterns of energy deficiency, evidenced by reduced activitiesof complexes I, II and/or IV after 30 days of hyperglycemia in heart, skeletal muscle and liver; moreover,cardiac tissue was found to be the most sensitive to the diabetic