Altered Expression of Matrix Metalloproteinases and Their TissueInhibitors as Possible Contributors to Corneal Droplet Formationin Climatic Droplet Keratopathy.

HOLOPAINEN JM; SERRA HM; SANCHEZ MC,; SORSA T; ZALENTEIN WN; BARCELONA PF,; MOILANEN JAO; TERVAHARTIALA T; TERVO TMT; CAFARO TA; VIRTANEN I; URRETS-ZAVALIA EA; BHATTACHARYA SK; URRETS-ZAVALIA JA

ACTA OPHTHALMOLOGICA

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2009 p. 123 - 123

1755-375X

limatic droplet keratopathy (CDK) is an acquired corneal disease characterized by progressive scarring of the cornea. In several corneal diseases, matrix metalloproteinases (MMPs) are upregulated during the degradation of epithelial and stromal tissues. We investigated the levels, degree of activation and molecular forms of MMP-2, MMP-9, MMP-8 and MMP-13 and their tissue inhibitors TIMP-1 and TIMP-2 in tear fluid of patients with CDK.Seventeen CDK patients and 10 controls living in Argentine Patagonia received a complete eye examination, and MMPs and TIMP-1/2 were determined by immunofluorometric assay (IFMA), gelatin zymography and quantitative Western immunoblot analysis in tear samples.The MMPs were detected mostly in their latent forms. The levels of MMP-9 and MMP-2 were found to be significantly elevated in CDK patients, whereas latent and active MMP-8 levels were significantly enhanced in controls. There was no significant difference in the level of MMP-13. TIMPs were found as p