Structural evaluation of plasma 2-Macroglobulin in acute pancreatitis

DE LORENC L, BISARO; RAMOS AM,; SANCHEZ, MARIA CECILIA; MONTENEGRO R,; CHIABRANDO GA,

CLINICAL CHEMISTRY AND LABORATORY MEDICINE

WALTER DE GRUYTER & CO

Lugar: Berlin; Año: 2005 vol. 43 p. 1183 - 1183

1434-6621

n this work we evaluate the proteolytic state of plasma a2-macroglobulin in acute pancreatitis. In addition, the plasma activity of matrix metalloproteinase-2(MMP-2), MMP-9 and serine proteinases were analyzed. A total of 33 patients with acute pancreatitiswere studied, of whom 16 were diagnosed as havingmild and 17 as having severe acute pancreatitis. In thelatter group, three patients progressed to multi-organfailure and died as a consequence of these complications. The proteolytic fragmentation of a2-macroglobulin was evaluated by Western blotting, whereasthe plasma activity of MMP-2, MMP-9 and serine proteinases was evaluated by gelatin zymography.Enhanced fragmentation of a2-macroglobulin wasdetected in severe acute pancreatitis patients withmultiple organ failure and lethal complications. In thissame patient group, increased plasma activity of theactive forms of MMP-2 and MMP-9, as well as serineproteinases, was apparent. In addition, we demonstrate that chymotrypsin-like protei