Panretinal photocoagulation (PRP) reduces the incidence of severe visual loss in proliferative diabetic retinopathy (PDR). The aim of the study
was to determine the effect of PRP on the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9, and also on the a2-Macroglobulin (a2M)
proteolytic state in the vitreous of eyes with PDR. Vitreous samples were obtained from patients undergoing vitrectomy for the treatment of
retinal diseases: 17 with PDR and eight with idiopathic macular hole (MH). Qualitative evaluation of the MMP-2 and MMP-9 activation status
was performed by gelatin zymography and quantitative assay was carried out for vitreous total protein content and a2M. The proteolytic state of
proteolytic state in the vitreous of eyes with PDR. Vitreous samples were obtained from patients undergoing vitrectomy for the treatment of
retinal diseases: 17 with PDR and eight with idiopathic macular hole (MH). Qualitative evaluation of the MMP-2 and MMP-9 activation status
was performed by gelatin zymography and quantitative assay was carried out for vitreous total protein content and a2M. The proteolytic state of
a2-Macroglobulin (a2M)proteolytic state in the vitreous of eyes with PDR. Vitreous samples were obtained from patients undergoing vitrectomy for the treatment of
retinal diseases: 17 with PDR and eight with idiopathic macular hole (MH). Qualitative evaluation of the MMP-2 and MMP-9 activation status
was performed by gelatin zymography and quantitative assay was carried out for vitreous total protein content and a2M. The proteolytic state of
a2M. The proteolytic state ofa2M was evaluated by Western blotting
2M was evaluated by Western blotting