CB-P18.
LRP1 EXPRESSION AND FUNCTION IN HUMAN
BLOOD PERIPHERAL MONOCYTE (BPM)
SUBPOPULATIONS
Ferrer DG, Jaldin-Fincati JR, Sanchez MC, Chiabrando GA.
CIBICI-CONICET, Depto Bioquímica Clínica Facultad de
Ciencias Químicas, Univ Nac Córdoba. E-mail:
dferrer@fcq.unc.edu.ar
Alpha2-Macroglobulin ( 2M*) is recognized by LDL receptorrelated
protein 1 (LRP1), and induces the cell migration of
macrophages. BPM are involved in the development of
atherosclerosis. In human, at least three subpopulations of BPM
may be identified (classical, intermediate and non-classical). LRP1
it has been associated with atherosclerotic processes. The LRP1
expression and function in BPM subpopulations is not well
established. By flow cytometry and confocal microscopy we
characterized the LRP1 expression in BPM subpopulations. By
two-dimensional wound scratch assay we examined the cell
migration of each BPM subpopulation isolated by cell sorter and
cultured in the presence of 2M*. LRP1 was three-fold more
expressed in classical than in intermediate and non-classical BPM
subpopulations. In classical BPM subpopulation, LRP1 was
mainly localized at perinuclear regions, increasing the cell surface
expression under 2M* stimulation for 30 min. In this time, the
classical BPM also formed cellular protrusions with accumulation
of F-actin and LRP1, which correlated with an increased cell
migration. Thus, our data demonstrate that classical BPM
subpopulation are induced to migrate by the 2M*/LRP1
interaction, which may be involved with the development of
