Requirement for p115 at a pre-Golgi VTC stage

ALVAREZ, C; FUJITA, H; HUBBARD, A; SZTUL, E

ROCKEFELLER UNIV PRESS

Lugar: New York; Año: 1999 vol. 147 p. 1205 - 1205

he membrane transport factor p115 functionsin the secretory pathway of mammalian cells. Usingbiochemical and morphological approaches, weshow that p115 participates in the assembly and maintenanceof normal Golgi structure and is required for ERto Golgi traffic at a pre-Golgi stage. Injection of antibodiesagainst p115 into intact WIF-B cells causedGolgi disruption and inhibited Golgi complex reassemblyafter BFA treatment and wash-out. Addition ofanti?p115 antibodies or depletion of p115 from aVSVtsO45 based semi-intact cell transport assay inhibitedtransport. The inhibition occurred after VSV glycoprotein(VSV-G) exit from the ER but before its deliveryto the Golgi complex, and resulted in VSV-Gprotein accumulating in peripheral vesicular tubularclusters (VTCs). The p115-requiring step of transportfollowed the rab1-requiring step and preceded theCa21-requiring step. Unexpectedly,