Spatial-temporal study of Rab1b dynamics and function at the ER-Golgi interface

HERNÁN MARTINEZ; GARCIA IA; SAMPIERI, L; ALVAREZ, C

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2016

1932-6203

!--[if gte mso 9]> 800x600 The GTPase Rab1b is involved in ER to Golgi transport, withmultiple Rab1b effectors (located at ERES, VTCs and the Golgi complex) beingrequired for its function. In this study, we performed live-celldual-expression studies to analyze the dynamics of Rab1b and some effectorslocated at the ERES-Golgi interface. Rab1b occupied widely distributed mobile punctateand tubular structures, displaying a transient overlaps with its effectors and showingthat these overlaps occurred at the same time in spatially distinct steps of ERto Golgi transport. In addition, we assessed Rab1b dynamics during cargosorting by analyzing the concentration at ERES of a Golgi protei