Rab1 GTPase Modulates the expression of genes involved in protein trafficking
Romero, Nahuel; Dumur, Catherine; Monetta, Pablo M; Slavin Ileana and Alvarez, Cecilia
The GTPase Rab1 is essential for Endoplasmic Reticulum to Golgi transport. By using genome-wide microarray analysis on HeLa cells expressing Rab1 and mutants we have identified (SAIB 2005) specific Rab1 dependent changes of gene transcription. Results showed that about 300 probe sets were significantly regulated by expression of Rab1. Here, different non-related criterions were used to select interesting genes, from the reported microarray results, to be validated by quantitative R90
T-PCR (TaqMan). Genes that code for proteins involved in membrane traffic (6), transcription factors (2), and two proteins used as controls, were selected. Our results showed that quantitative RT-PCR results showed very good correlation with Affymetrix values (Pearson´s r >