Chemokine response induced by Chlamydia trachomatis in prostate derived CD45+ and CD45- cells.

MACKERN-OBERTI JP, BRESER ML, NUÑEZ N, MACCIONI M, RODRIGUEZ M, WANTIA N, ELEY A, MIETHKE T, RIVERO VE

BIOSCIENTIFICA LTD

Año: 2011 vol. 142 p. 427 - 427

he role of innate cells and their receptors within the male genital tract remains poorly understood. Much less is known about the relative contribution of different genital tract cells such as epithelial/stromal cells and resident leucocytes. In this study, we examined innate immune responses to Chlamydia trachomatis by prostate epithelial/stromal cells and prostate resident leucocytes. Murine prostate primary cultures were performed and leucocyte and epithelial/stromal cells were sorted based on surface protein expression of CD45 by magnetism-activated cell sorting or fluorescence-activated cell sorting. Prostate derived CD45- and CD45+ cells were infected with C. trachomatis and chemokine secretion assayed by ELISA. Similar experiments were performed using prostate CD45+ and CD45- cells from myeloid differentiation factor 88 (Myd88(-/-)) mice or toll-like receptor (Tlr2(-/-)) and Tlr4(mutant) double-deficient mice. Moreover, a TLR-signalling pathway array was used to screen changes