Efficacy of a cationic polymer as an adjunct to antibiotics with different target site against Pseudomonas aeruginosa

ROMERO, VERÓNICA L; ROSSET, CLARISA I.; MANZO RUBEN H.; ALOVERO, FABIANA L.

International Journal Of Pharmaceutical Sciences And Research (IJPSR) ISSN: 0975-8232

Society of Pharmaceutical Sciences and Research

Lugar: New Delhi; Año: 2015 p. 152 - 152

merging drug resistance, along with the few antibiotic discovery programs in the pharmaceutical industry has forced the scientific community to seek new alternatives. Numerous reports describe the antimicrobial properties of cationic polymers for pharmaceutical use. However, Eudragit E100® (Eu) has not been characterized in this sense. Recently, we described that Eu causes alterations in the bacterial envelopes of P.aeruginosa, but the polymer itself is not bactericidal. In this study we evaluate the effects, if any, of Eu on the antibacterial action of antibiotics with different site and spectrum of action: Ciprofloxacin (CIP) and Vancomycin (VAN) against P.aeruginosa. It is known that P.aeruginosa is outside of spectrum of action of VAN. In contrast, CIP is an effective antimicrobial against P.aeruginosa but growing resistance in clinical isolates has limited its use. Bactericidal activity of drug-containing Eu dispersions (Eu-CIP and Eu-VAN) was determined by time-kill assay a