Phototoxicity of cationic porphyrin against multi-drug resistant Pseudomonas aeruginosa

QUIROGA; EZEQUIEL D.; DURANTINI, EDGARDO; ALOVERO FABIANA L.

International Journal Of Pharmaceutical Sciences And Research (IJPSR) ISSN: 0975-8232

Society of Pharmaceutical Sciences and Research

Lugar: New Delhi; Año: 2015 p. 149 - 149

seudomonas aeruginosa is the cause of various infections. Amongst others, eye and ear infections or those in skin tissue and soft in burn patients represent a major therapeutic challenge. In recent years, photodynamic inactivation (PDI) has been proposed as an alternative treatment for localized bacterial infections in response to the problem of antibiotic resistance. The principle of PDI is to use a non-toxic dye or photosensitizer (PS) to produce reactive oxygen species (ROS) to cause damages on target bacteria while the PS is activated by a harmless visible light. The main purpose of this study was to explore the PDI effect of 5,10,15,20-tetra(4-N-methylpyridyl)porphyrin (TPMyP4+) on P.aeruginosa ATCC 27853 and a clinical isolate of MDR P.aeruginosa since reports of their effects against multi-drug resistant (MDR) bacteria are limited. The porphyrin was evaluated for different doses (2.5, 5, 10, 20 µM), 30 min of irradiation with visible light and cellular suspensions of ~ 1x106 C