Eudragit E100: Antibiofilm and Antivirulence Properties Allow Propose this Cationic Polymer as an Adjuvant to Conventional Antimicrobials in a Promising Approach against Pseudomonas aeruginosa Infections

ALOVERO, FABIANA L.

Proceedings International

Romanian ISSN Centre

Año: 2022 vol. 4 p. 88 - 88

seudomonas aeruginosa is an opportunistic pathogen that exhibits its pathogenesis property due to several factors, and antimicrobial resistance is a substantial problem in its treatment. In this scenario, treatments targeting bacterial virulence have gained considerable interest. Eudragit E100® (Eu) is a cationic copolymer not fully characterized by antimicrobial properties. Previously, we demonstrated that Eu enhanced the efficacy of various antimicrobials against P.aeruginosa (planktonic) without exhibiting bactericidal action in itself. Here, we investigate the ability of Eu to prevent biofilm formation and inhibit the production of relevant virulence factors of multi-resistant P.aeruginosa. Eu concentration-dependent reduction in biofilm biomass was evidenced by crystal violet staining assay. Furthermore, a significant reduction in the production of pyocyanin, pyorubin, and proteases was observed and in bacterial motility. Reduced pigmentation observed by extraction with chlorofor