Photodynamic hydrogel based on Carbomer-TMPyP complex: in vitro release and P.aeruginosa photoinactivation

ALOVERO, FABIANA L.

Proceedings International

Romanian ISSN Centre

Año: 2022 vol. 4 p. 109 - 109

hotodynamic antimicrobial chemotherapy is a proposed alternative for localized infections in the age of antibiotic resistance. While topical application of a photosensitizer followed by irradiation is a viable application due to the accessibility of the target site, the desing of an effective and safe delivery systems for the photosensitizer are nevertheless necessary. Previously, we designed hydrogels based on ionic complexes between Carbomer-974P and a cationic porphyrin, TMPyP, which showed slight lower photoinactivation of P.aeruginosa than TMPyP solution. Next, we present studies of in vitro release using Franz-type cells as well as the behavior exhibited in the interaction/association of TMPyP with bacterial cells analyzed by CLSM and FM.Release profiles fit better with the zero-order kinetic (R2=0.993) and hydrogel showed a release rate 6.5 times slower than TMPyP solution. Furthermore, a delay in the detection of fluorescent bacteria was observed in hydrogel-treated cultures