ALOVERO FABIANA DEL LUJAN
Congresos y reuniones científicas
Título:
EUDRAGIT E100 POTENTIATES ACTIVITY OF OFLOXACIN AGAINST FQ-RESISTANT Pseudomonas aeruginosa
Autor/es:
ROMERO, VERÓNICA L; MANZO, RUBEN H.; ALOVERO, FABIANA L.
Reunión:
Congreso; 40° Reunion Anual de Sociedad Argentina de Farmacología Experimental (SAFE),; 2008
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
Fluoroquinolones (FQs) resistance is a clinically
significant issue. Efforts to improve the efficacy of existing
FQs could be an alternative to restrict the development of
FQ-resistant bacteria prolonging their future utility.
Eudragit E100 (Eu) is a cationic polyelectrolyte able to
react with the acidic group of OFLOXACIN yielding
Eu-OFLOx complexes where OFLO neutralizes 20-50%
of the basic groups of Eu. The addition of a second
counterion (Cl-) turns the complexes water soluble giving
clear dispersions (Eu-Cl50-OFLOx) with positive
electrokinetic potential () and pHs 6.4-6.5. The activity
against FQ-R P.aeruginosa was enhanced exhibiting
MICs two or four-fold lower than free OFLO and improved
bactericidal effect. Eu-Cl50 without OFLO exhibits an initial antibacterial effect reversible along the time while
Eu-Cl50-OFLOx exhibits enhanced effect leading
complete bacterial eradication at concentrations or time
assays where free OFLO does not show it. This
behaviour could be attributed to the improved drugbacterial
cell interaction, topic in which the high of Eu-
Cl50-OFLOx could play a preponderant role.