Structural, physicochemical and biological characterization of chloramphenicol multicomponent complexes.

JUAN PABLOCERUTTI; VIRGINIA AIASSA; MARIANA ADELA FERNÁNDEZ; MARCELA LONGHI; MARIO QUEVEDO; ARIANA ZOPPI

JOURNAL OF MOLECULAR LIQUIDS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2021 vol. 331 p. 115761 - 115761

0167-7322

he aimof this studywas to improve the solubility, antibiofilm activity and safety profile of chloramphenicol (CP)by the preparation of multicomponent complexes with γ-cyclodextrin (γ-CD) and N-acetylcysteine (NAC). Thephase solubility and isothermal titration calorimetry (ITC) studies suggested the complex formation and revealedthe solubility of CP is enhanced during the complexation. The most important driving forces of the formation ofthe complexes were determined by molecular modeling techniques and ITC. Complexation evidence was alsoprovided by data from NMR experiments. The solid-state materials were prepared by physical mixture andfreeze-drying methods and subjected to physicochemical characterization using scanning electron microscopy,electron probe microanalysis, thermoanalytical methods and powder X-Ray diffraction. The ability of the materialsto decrease the cellular activity of Staphylococcus aureus biofilmswas studied by XTT assay. Furthermore, reactiveoxygen spe