An Alkaloid Extract Obtained from Phlegmariurus saururus Induces Neuroprotection after Status Epilepticus

DANELON V,; MONTRULL L,; VALLEJO M; CABRERA J.L; AGNESE, A.M.; ORTEGA MG; MASCO D

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2017 vol. 34 p. 212 - 212

0944-7113

ackground: The brain is exposed to many excitotoxic insults that can lead to neuron damage. Among these, Epilepsy is a neurological disease that affects a large percentage of the world population and is commonly associated with cognitive disorders and excitotoxic neuron death. Most experimental strategies are focused on preventing Status Epilepticus (SE), but once it has already occurred, the key question is whether it is possible to save neurons.Purpose: The aim of this study was to determine if a purified alkaloid extract (AE) obtained from Phlegmariurus saururus, a genus of Lycophyte plants, sometimes known as firmosses or fir club mosses, could induce neuroprotection following SE. Methods: In vitro and an in vivo approaches were used for this purpose. Protein levels were measured by western blot. Neuronal death analysis was performed by calcein-ethidium staining (in vitro experiments) and by Fluoro Jade B staining for the in vivo experiments.Results: The effect of AE inhippocampal