Interactions of a prenylated flavonoid from Dalea elegans with fluconazole against azole- resistant Candida albicans

BARCELO S; PERALTA M; CALISE M,; FINCK, SOLEDAD; ORTEGA MG; DIEZ R.A.,; CABRERA J; PEREZ CRISTINA

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2017

0944-7113

ackgound: The prenylated flavonoid 2?, 4?-dihydroxy-5?-(1???, 1???-dimethylallyl)-8-prenylpinocembrin (8PP, formerly 6PP) shows antifungal activity, inhibits rhodamine 6G efflux and reverses fluconazole (FCZ)resistance in azole-resistant Candida albicans overexpressing cdr1, cdr2 and mdr1 transporters.Purpose and design: In this paper, we tried to characterize 8PP in vitro interactions on the cell growth and lethality of C. albicans. We also initiated preliminary in vivo toxicological studies on mice.Methods: The effects of 8PP and FCZ on cell growth and viability of C. albicans were evaluated by CLSI standards. The checkerboard assay was used to search for interactions on cell growth. The time-kill assay was used to study fungicidal effects. Acute toxicity was evaluated at a single dose schedules.Results: From the checkerboard design, and using a starting inoculum of 103 CFU/ml, the fractional inhibitory concentration (FIC) of FCZ and 8PP could be determined as 0.11 and 0.50, respect