Antifungal activity of a prenylated flavonoid from Dalea elegans against Candida albicans biofilms

MARIANA PERALTA,.; MARÍA ANGEL DA SILVA; MARÍA GABRIELA ORTEGA; CABRERA J.L.,; PARAJE MG

ELSEVIER GMBH

Año: 2015 vol. 22 p. 975 - 975

ackground: The continuing emergence of infections with antifungal resistant Candida strains requires a constant search for new antifungal drugs, with the plant kingdom being an important source of chemical structures.Purpose: The present study investigated the antifungal effect of 2,4-dihydroxy-5-(1,1-dimethylallyl)-8-prenylpinocembrin (8PP, formerly 6PP), a natural prenylflavonoid, on Candida albicans biofilms, and compared this with an azole antifungal (fluconazole) by studying the cellular stress and antioxidant response. Study design/methods: The fluconazole sensitive (SCa) and azole-resistant (RCa) C. albicans strains were used,with biofilm formation being studied using crystal violet (CV) and confocal scanning laser microscopy (CSLM).The minimal inhibitory concentration for sessile cells (SMIC) was defined as the concentration of antifungal that caused a 50% (SMIC 50) and 80% (SMIC 80) reduction of treated biofilms. The reactive oxygen species (ROS) were detected by the reductio